VNO Atlas Figure 3

This file contains statistics and figures from the paper, “Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal organ at Single Cell Resolution” by Max Hills Jr., Limei Ma, Ai Fang, Thelma Chiremba, Seth Malloy, Allison Scott, Anoja Perera, and C. Ron Yu.

The paper can be found here:

Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal organ at Single Cell Resolution

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Molecular cascades separating the neuronal lineages

PCA plot of V1R and V2R pseudotime principal curves across cell types

Cell density plots across pseudotime for P14 and P56 mice

Summary of Kolmogorov-Smirnov Test

    Two-sample Kolmogorov-Smirnov test

data:  p14.v1r.pseudotime$v1r.pseudotime and p56.v1r.pseudotime$v1r.pseudotime
D = 0.21438, p-value < 2.2e-16
alternative hypothesis: two-sided

    Two-sample Kolmogorov-Smirnov test

data:  p14.v2r.pseudotime$v2r.pseudotime and p56.v2r.pseudotime$v2r.pseudotime
D = 0.10572, p-value = 1.892e-13
alternative hypothesis: two-sided

Heatmap showing expression in pseudotime for genes that differentially expressed between the V1R and V2R lineages. Heat indicates Z-score values

Zoomed in UMAP of cell types early in the neuronal lineage

Feature plots for select genes expressed early in the neuronal lineage

Violin plot of normalized expression for Neurog1 and Neurod1, split by cell type

UMAP of INP, iVSN, iOSN, and mOSN cell types

Normalized expression of candidate genes for V1R/V2R/OSN lineage determination - V1R expressing genes

Normalized expression of candidate genes for V1R/V2R/OSN lineage determination

Feature plots of normalized gene expression for early differentially expressed genes between V1R, V2R, and OSN lineages

Feature plots of normalized expression for Notch1 and Dll4